Journal: Biomolecules

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

doi: 10.3390/biom16040582

Figure Lengend Snippet: Isoproterenol depletes intracellular iron in H9c2 cells. Analysis of intracellular iron levels from the cells challenged with isoproterenol and/or deferoxamine. Data are expressed as mean ± SEM; (*** p < 0.001 vs. control).

Article Snippet: Rat embryonic heart-derived H9c2 cells (from ATCC-Massanas, VA, USA; CRL-1446) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; 4.5 g/L glucose), supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C in a humidified incubator with 95% air and 5% CO 2 [ ].

Techniques: Control

Journal: Biomolecules

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

doi: 10.3390/biom16040582

Figure Lengend Snippet: Isoproterenol modifies iron metabolism-related molecules in H9c2 cells. Analysis of mRNA ( a , c , e , f ) and protein levels ( b , d , g , h ) of IRP1 ( Aco1 ) ( a , b ), IRP2 ( Ireb2 ) ( c , d ), Fth1 ( e ), Ftl1 ( f ) and FERRITIN ( g ). Representative Western blot image of all the analyzed proteins ( h ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or by ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. ISO; && p < 0.01 vs. DEF).

Article Snippet: Rat embryonic heart-derived H9c2 cells (from ATCC-Massanas, VA, USA; CRL-1446) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; 4.5 g/L glucose), supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C in a humidified incubator with 95% air and 5% CO 2 [ ].

Techniques: Western Blot, Control

Journal: Biomolecules

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

doi: 10.3390/biom16040582

Figure Lengend Snippet: Isoproterenol modifies molecules involved in intracellular iron uptake in H9c2 cells. Analysis of mRNA ( a , c ) and protein ( b , d , e ) levels of TFRC ( a , b ) and DMT1 ( c , d ). Representative Western blot image of all the analyzed proteins ( e ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or ACTIN protein levels and expressed as mean ± SEM; (** p < 0.01 and *** p < 0.001 vs. control).

Article Snippet: Rat embryonic heart-derived H9c2 cells (from ATCC-Massanas, VA, USA; CRL-1446) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; 4.5 g/L glucose), supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C in a humidified incubator with 95% air and 5% CO 2 [ ].

Techniques: Western Blot, Control

Journal: Biomolecules

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

doi: 10.3390/biom16040582

Figure Lengend Snippet: Isoproterenol modifies molecules involved in intracellular iron release in H9c2 cells. Analysis of mRNA ( a , c ) and protein levels ( b , d , e ) of FPN ( a , b ) and HEPCIDIN ( c , d ). Representative Western blot image of all the analyzed proteins ( e ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; ### p < 0.001 vs. ISO and &&& p < 0.001 vs. DEF).

Article Snippet: Rat embryonic heart-derived H9c2 cells (from ATCC-Massanas, VA, USA; CRL-1446) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; 4.5 g/L glucose), supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C in a humidified incubator with 95% air and 5% CO 2 [ ].

Techniques: Western Blot, Control

Journal: Biomolecules

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

doi: 10.3390/biom16040582

Figure Lengend Snippet: Isoproterenol modifies the expression of molecules involved in mitochondrial iron uptake and release in H9c2 cells. Analysis of Ftmt, Mfrn1, Mfrn2, Abcb7 and Abcb8 mRNA ( a , c , e , g , i ) and protein levels ( b , d , f , h , j ). Representative Western blot image of all the analyzed proteins ( k ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or by ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; # p < 0.05 and ## p < 0.01 vs. ISO).

Article Snippet: Rat embryonic heart-derived H9c2 cells (from ATCC-Massanas, VA, USA; CRL-1446) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; 4.5 g/L glucose), supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C in a humidified incubator with 95% air and 5% CO 2 [ ].

Techniques: Expressing, Western Blot, Control

Journal: Biomolecules

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

doi: 10.3390/biom16040582

Figure Lengend Snippet: Isoproterenol downregulates mitofusin 1 and 2 expressions in H9c2 cells. Analysis of Mfn1 and Mfn2 mRNA ( a , c ) and protein levels ( b , d ). Representative Western blot image of all the analyzed proteins ( e ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05 and *** p < 0.001 vs. control; # p < 0.05 vs. ISO).

Article Snippet: Rat embryonic heart-derived H9c2 cells (from ATCC-Massanas, VA, USA; CRL-1446) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; 4.5 g/L glucose), supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C in a humidified incubator with 95% air and 5% CO 2 [ ].

Techniques: Western Blot, Control